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Handy PEA 連續(xù)激發(fā)式熒光儀
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Handy PEA植物效率分析儀 能夠完整測(cè)定葉綠素OJIP熒光誘導(dǎo)動(dòng)力學(xué)曲線的連續(xù)激發(fā)式熒光儀 主要應(yīng)用范圍: 植物生理學(xué)、植物生態(tài)學(xué)、農(nóng)學(xué)、園藝學(xué)、林學(xué)、環(huán)境科學(xué)、植物病理學(xué)、藻類生物學(xué)等領(lǐng)域。 測(cè)定參數(shù): 全新軟件可直接計(jì)算出Fo,Fm,Fv,Fv/Fm,Ft,FJ,FI,FP,Tm, ψo,φEo,φDo,Vt,VJ,WK,PIABS,PICS,ABS/RC,TRo/RC,ETo/RC,DIo/RC,RC/CSo,RC/CSM等五十個(gè)葉綠素?zé)晒鈪?shù)。準(zhǔn)確記錄葉綠素?zé)晒庹T導(dǎo)動(dòng)力學(xué)曲線的快相部分,每秒鐘可以連續(xù)記錄10萬(wàn)次熒光蹤跡數(shù)據(jù),在1秒鐘內(nèi)完整測(cè)定葉綠素的OJIP熒光誘導(dǎo)動(dòng)力學(xué)曲線。 全新的葉綠素?zé)晒饫碚撛忈?/span> Handy PEA植物效率分析是葉綠素?zé)晒鈨x的一種,屬于連續(xù)激發(fā)式熒光儀。Handy PEA是PEA植物效率分析儀系列儀器中功能強(qiáng)大,價(jià)格中等,國(guó)內(nèi)用戶數(shù)量非常龐大。 與傳統(tǒng)的脈沖調(diào)制式熒光儀不同,Handy PEA具有超高的時(shí)間分辨率,分辨率高達(dá)105,是能夠準(zhǔn)確記錄O-J-I-P快速葉綠素?zé)晒庹T導(dǎo)動(dòng)力學(xué)曲線的儀器。幾乎所有植物(>99%)的OJIP曲線可以在1秒鐘內(nèi)完成準(zhǔn)確測(cè)定。OJIP曲線的數(shù)據(jù)分析使用基于JIP-Test的分析方法,該方法由PEA植物效率分析儀的創(chuàng)始人Strasser教授創(chuàng)建。 通過(guò)OJIP曲線我們可以準(zhǔn)確的分析光合機(jī)構(gòu)在逆境下,是否受到傷害?光合機(jī)構(gòu)哪個(gè)部位受到傷害?受傷害的程度多大?既可以進(jìn)行定性分析,也可以對(duì)傷害程度進(jìn)行定量分析。 儀器發(fā)明人Strasser教授簡(jiǎn)介: R.T.Strasser是瑞士日內(nèi)瓦大學(xué)教授,國(guó)際禁用詞匯的光合作用研究專家,是日內(nèi)瓦大學(xué)生物能學(xué)和微生物學(xué)重點(diǎn)實(shí)驗(yàn)室的主任,聯(lián)合國(guó)地中海沙漠植被恢復(fù)項(xiàng)目評(píng)估專家組成成員,主要從事光合作用和光合模擬研究。 高等植物,藻類通用的熒光儀 圖5 用于高等植物的Handy PEA探頭 圖6 用于藻類樣品的Handy PEA探頭 Handy PEA植物效率分析儀,標(biāo)配高等植物探頭,非常方便的進(jìn)行葉片O-J-I-P快速葉綠素?zé)晒庹T導(dǎo)動(dòng)力學(xué)曲線的測(cè)定,也可以選配藻類樣品探頭,測(cè)定藻類的OJIP曲線。 測(cè)定高等植物的探頭測(cè)定不受葉面積的影響,HPEA/LC暗適應(yīng)夾(葉夾),測(cè)定直徑為4mm,對(duì)于小于4mm的植物葉片,Handy PEA通過(guò)數(shù)據(jù)標(biāo)準(zhǔn)化進(jìn)行處理,不影響數(shù)據(jù)的準(zhǔn)確性。 測(cè)定藻類的樣品探頭HPEA/LPA2具有極高的分辨率,很低的藻類樣品即可進(jìn)行準(zhǔn)確的測(cè)定,如硅藻,在肉眼幾乎分辨不出存在的情況下,即可測(cè)定完整準(zhǔn)確的OJIP曲線。 超級(jí)便攜的用戶體驗(yàn) 圖7 便攜性優(yōu)異的Handy PEA用于田間測(cè)定 Handy PEA植物效率分析儀全套設(shè)備重量不足1kg,有背帶,方便進(jìn)行野外操作,即使是女科研人員也可以一個(gè)人完成所有的操作。 測(cè)定數(shù)據(jù)快速,儀器標(biāo)配20個(gè)暗適應(yīng)夾(6g),可以一次進(jìn)行至少20個(gè)樣品的暗適應(yīng)處理,測(cè)定一個(gè)樣品的時(shí)間為2s左右?梢栽跇O短的時(shí)間內(nèi)完成大量的測(cè)定,獲取大量的有價(jià)值的數(shù)據(jù)。在大數(shù)據(jù)時(shí)代,Handy PEA必然會(huì)進(jìn)一步體現(xiàn)出它的強(qiáng)大功能。 功能強(qiáng)大的軟件
圖 8 友好的Handy PEA軟件界面 軟件界面友好,操作簡(jiǎn)單,具有篩選的功能,可以在大數(shù)據(jù)時(shí)代,用于優(yōu)良品種、抗性品種的篩選。進(jìn)行分組、分類、平均、規(guī)律等分析。 強(qiáng)大的售后服務(wù)團(tuán)隊(duì) 在駱兵博士帶領(lǐng)的所有的售后服務(wù)工程師都具有碩士研究生及其以上學(xué)歷,并具有使用該儀器做實(shí)驗(yàn)3年以上的實(shí)戰(zhàn)經(jīng)驗(yàn)。 售后服務(wù)既可以提供儀器的理論講座,也可以專門(mén)介紹在儀器使用過(guò)程中可能出現(xiàn)的錯(cuò)誤及避免方案,還可以介紹大量的寶貴經(jīng)驗(yàn),或者實(shí)測(cè)用戶現(xiàn)有的材料,進(jìn)行數(shù)據(jù)分析,幫助用戶在短時(shí)間內(nèi)將儀器用于科研工作。 數(shù)量巨大的文獻(xiàn)支持(禁用詞匯新文獻(xiàn)請(qǐng)查看微信公眾號(hào)“漢莎科技集團(tuán)”) 2015年 Agrawal D, Jajoo A. Investigating primary sites of damage in photosystem II in response to high temperature[J]. Indian Journal of Plant Physiology, 2015, 20(4): 304-309. Akhtar S S, Andersen M N, Liu F. Residual effects of biochar on improving growth, physiology and yield of wheat under salt stress[J]. Agricultural Water Management, 2015, 158: 61-68. Akhter A, Hage-Ahmed K, Soja G, et al. 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