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多通道線粒體呼吸解決方案

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Oxytherm+多通道線粒體呼吸測(cè)定系統(tǒng)

先進(jìn)的Clark氧電極系統(tǒng)——研究線粒體呼吸的科研利器

實(shí)時(shí)監(jiān)測(cè)反應(yīng)體系中溶解氧的濃度及變化，可多通道（最大8通道）同時(shí)檢測(cè)多個(gè)樣品

  • 研究線粒體的I態(tài)、II態(tài)、III態(tài)、IV態(tài)呼吸，計(jì)算呼吸控制率及P/O比等；

  • 研究肌肉、肝臟、大腦器官組織等細(xì)胞、細(xì)菌、酵母、藻類等微生物的呼吸（耗氧）速率；

  • 研究呼吸途徑（抗氰呼吸、細(xì)胞色素氧化酶途徑、糖酵解途徑等）；

  • 研究有氧參與的酶促反應(yīng)(生物反應(yīng)）速率，有氧參與的化學(xué)反應(yīng)；

  • 研究某些化學(xué)合成物質(zhì)的放氧速率。

1585277496380318.jpg系統(tǒng)特點(diǎn)

  • 24位高分辨率10-3pmol·ml-1

  • 電子精確控溫(精度±0.5℃）

  • 多通道實(shí)時(shí)顯示數(shù)據(jù)（1～8通道可選）

  • 樣品用量少0.2～2.5ml

  • 能與H+電極同時(shí)測(cè)定

3.jpg氧電極用于測(cè)定酶活性Sevier C S，et al.Cell,2007,129(2):333-44

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線粒體狀態(tài)呼吸和呼吸控制率

Papathanasiou S, Rickelt S, Soriano M E, et al. Tumor necrosis factor-α confers cardioprotection through ectopic expression of keratins K8 and K18[J]. Nature Medicine,2015,21(9):1076.

1585277564645187.jpg氧電極用于抗體免疫研究llani T, et al. Science, 2013, 341(6141):74 

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氧電極用于測(cè)定細(xì)胞呼吸Zhang H, et al. Cancer Cell, 2007, 11(5):407

部分應(yīng)用文獻(xiàn)

Cipolat S, Rudka T, Hartmann D, et al. Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling[J]. Cell, 2006, 126(1):163-175.

Chen H, Vermulst M, Wang Y E, et al. Mitochondrial fusion is required for mtDNA stability in skeletal muscle and tolerance of mtDNA mutations[J]. Cell, 2010, 141(2): 280-289.

Wang G, Chen HW,Oktay Y, et al. PNPASE regulates RNA import into mitochondria[J]. Cell, 2010, 142(3): 456-467.

Lee H Y, Choi C S, Birkenfeld A L, et al. Targeted expression of catalase to mitochondria prevents age-associated reductions in mitochondrial function and insulin resistance[J]. Cell metabolism, 2010, 12(6): 668-674.

De Silva D, Fontanesi F, Barrientos A. The DEAD box protein Mrh4 functions in the assembly of the mitochondrial large ribosomal subunit[J]. Cell metabolism, 2013, 18(5): 712-725.

Acetoze G, Weber K L, Ramsey J J, et al. Relationship between Liver Mitochondrial Respiration and Proton Leak in Low and High RFI Steers from Two Lineages of RFI Angus Bulls[J]. International Scholarly Research Notices, 2015, 2015.

Samaiya P K, Krishnamurthy S. Characterization of mitochondrial bioenergetics in neonatal anoxic model of rats[J]. Journal of bioenergetics and biomembranes, 2015, 47(3): 217-222.

Lai L, Jin J C, Xu Z Q, et al. Spectroscopic and Microscopic Studies on the Mechanism of Mitochondrial Toxicity Induced by CdTe ODs Modified with Different Ligands[J]. The Journal of membrane biology, 2015: 1一14.

Jacoby R P, MiIlarA H, Taylor N L. Assessment of Respiration in Isolated Plant Mitochondria Using Clark-Type Electrodes[J]. Plant Mitochondria: Methods and Protocols, 2015: 165-185.

Mitochondrial Uncoupling and Glutathione Redox Cycling, with Protection Against Oxidant Injury in H9c2 Cells[J]. Food & Function, 2015, 6(2): 549-557.
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